ABSTRACT
Objective To compare the chemical composition and the pharmaceutical effect of anti-heart failure of wild commodity Astragali Radix (WAR) and cultivated Astragali Radix (CAR). Methods The contents of flavonoids and saponins in Astragali Radix samples were determined by HPLC-UV-ELSD. The intervention effects of wild commodity and cultivated Astragali Radix on traditional efficacy index and metabolomics were compared in rat model with heart failure induced by adriamycin. Results The contents of total flavonoids, saponin I, and saponin III in WAR were significantly higher than those in CAR, but the content of total saponins was the equivalent. Pharmacodynamics experimental results showed that both WAR and CAR could improve the general situation in rats, histopathology, cardiac function parameters, serum biochemical indicators, and BNP. However, compared with the model group, the intervention effects of the WAR on cardiac function parameters (EF, FS, and LVIDs), biochemical markers (CK) and serum BNP content were superior to the CAR, and showed significant differences. The principal component analysis showed that the metabolic profiles of the normal control group and the model group were clearly distinguished. A total of 14 potential biomarkers associated with heart failure were identified, which could be adjusted to varying degrees in WAR and CAR, of which 2-hydroxyisobutyric acid, acetone, pyruvate, and formate were significantly changed in the WAR group. Conclusion In this study, the intervention of WAR in heart failure rats was significantly better than that of CAR, which provided a scientific basis for the establishment of specification grading, the clinical rational drugs use, and the classification of Astragali Radix by pharmaceutical companies for Astragali Radix.
ABSTRACT
Currently, the specification grading standard for Astragali Radix can not accurately reflect growth years. The aim of this study is to identify the growth ring number of different parts of 1 to 6 year Hengshan imitative wild culture Astragali Radix, in order to get a different absolute growth years, to classify the accumulation rules of the content of flavonoids and saponins, and to lay the foundation for evaluating quality of Astragali Radix. Observing growth ring numbers of 1-6 years Astragali Radix by means of hand sections and paraffin sections in the study, and analyzing the number of different growth years and different diameter. At the same time, HPLC-UV-ELSD was used to analyze the 12 index components of the samples with absolute growth years of 2 to 6 years. The results indicated that the growth ring number excepting hollow part is consistent with the actual growth period of Astragali Radix and the number of growth rings gradually decreased from the upper to lower. The results of HPLC-UV-ELSD determination showed that the saponins content of 3-year-old Astragali Radix was the highest while the flavonoids content of the 4-year-old reached the maximum. The study provided the basis for foundation of the specification grading standard for Astragali Radix and clinical rational use drug.